Description
Agilent RNA kits are used for the automated quality control analysis of mRNA and total RNA, including formalin-fixed, paraffin-embedded (FFPE) RNA quality assessment and in vitro transcribed (IVT) mRNA sizing, purity, and integrity measurement. Quality RNA is crucial to many applications, including mRNA vaccine development, qPCR, NGS, microarray analysis, and northern blot analysis.
Agilent ProSize data analysis software calculates an RNA quality number (RQN), providing a quantitative total RNA quality metric for downstream applications. The excellent resolution allows users to easily distinguish between RNA degradation and small RNA.
Features - Reliable sizing - Measure accurate and precise sizing of RNA samples from 200 to 6,000 nt using the standard method, or through 9,000 nt with the extended method
- Broad quantification range - Analyze samples of different concentrations ranging from 5 to 500 ng/µL with the RNA kit, or from 50 to 5,000 pg/µL with the HS RNA kit
- Minimized sample dilutions - Choose the kit that fits the right concentration range for your needs and thereby avoid sample dilutions
- Objective quality metrics - Determine the level of degradation of total RNA samples by the RQN ranging from 1 to 10, assess the percentage purity of mRNA samples, and calculate DV200 (the percentage of fragments larger than 200 nt) for FFPE RNA samples
- Low sample consumption - Use only 2 µL of sample to obtain accurate results
- Specific RNA analysis modes - Analyze RNA samples of different origin like eukaryotic, prokaryotic, plant, and ribodepleted RNA by selecting the corresponding method in ProSize data analysis software